The enzyme can be reconstituted in 20 mM sodium phosphate buffer, pH 7.0, with 0.15 M sodium chloride.
B4186
Butyrylcholinesterase human
vial of ≥4 units
Synonym(s):
Acylcholine acyl-hydrolase, Choline esterase, butyryl, Pseudocholinesterase
Sign Into View Organizational & Contract Pricing
Select a Size
1 VIAL
€799.00
All Photos(4)
Select a Size
Change View
1 VIAL
€799.00
About This Item
Recommended Products
form
powder
Quality Level
specific activity
≥50 units/mg protein
packaging
vial of ≥4 units
UniProt accession no.
storage temp.
2-8°C
Gene Information
human ... BCHE(590)
Looking for similar products? Visit Product Comparison Guide
Application
BchE, from Sigma, has been used to determine butyrylcholinesterase inhibition by various test compounds [1].
Butyrylcholinesterase human has been used to study its role as a modulator of pathological features of Alzheimer′s disease (AD) in vivo.[2]
Biochem/physiol Actions
Butyrylcholinesterase (BChE) is a serine hydrolase that shares substantial structural similarities with acetylcholinesterase (AchE) but has different substrate and inhibitor specificities. BChE is found in the serum, hemopoietic cells, liver, lung, heart and the central nervous system of vertebrates.
Butyrylcholinesterase (BChE) is a serine hydrolase that shares substantial structural similarities with acetylcholinesterase (AchE) but has different substrate and inhibitor specificities[3]. It is found in the serum, hemopoietic cells, liver, lung, heart and the central nervous system of vertebrates.[4] BChE hydrolyzes large esters of choline such as butyrylcholine and benzoylcholine. Selective inhibition of BChE activity can be used in the detection of organophosphates.[5] BchE may be used to treat organophosphate toxicity. It is a critical target for Alzheimer′s disease (AD) therapy.[6]
Unit Definition
One unit will hydrolyze 1.0 μmole of butyrylcholine to choline and butyrate per min at pH 8.0 at 37 °C. The activity obtained using butyrylcholine as substrate is ~2.5 times that obtained using acetylcholine.
Analysis Note
Starting material individually donor tested and found negative for HIV I & II antibodies, Hepatitis B surfaceantigen, and Hepatitis C antibodies.
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Resp. Sens. 1
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Choose from one of the most recent versions:
Certificates of Analysis (COA)
Lot/Batch Number
Don't see the Right Version?
If you require a particular version, you can look up a specific certificate by the Lot or Batch number.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Customers Also Viewed
Cholinesterases and engineered mutants for the detection of organophosphorus pesticide residues
Xu YL, et al.
Sensors, 18(12), 4281-4281 (2018)
The apolipoprotein E varepsilon4 allele plays pathological roles in AD through high protein expression and interaction with butyrylcholinesterase
Darreh-Shori T, et al.
Neurobiology of Aging, 32(7), 1236-1248 (2011)
Butyrylcholinesterase: an important new target in Alzheimer's disease therapy
Greig NH, et al.
International Psychogeriatrics / IPA, 14(S1), 77-91 (2002)
The key role of butyrylcholinesterase during neurogenesis and neural disorders: an antisense-5? butyrylcholinesterase-DNA study
Mack A and Robitzki A
Progress in Neurobiology, 60(6), 607-628 (2000)
Huan-Huan Li et al.
Journal of enzyme inhibition and medicinal chemistry, 37(1), 2099-2111 (2022-07-29)
Novel scaffolds are expected to treat Alzheimer's disease, pyrazole-5-fluorosulfates were found as selective BuChE inhibitors. Compounds K1-K26 were assayed for ChE inhibitory activity, amongst them, compound K3 showed potent BuChE and hBuChE inhibition (IC50 = 0.79 μM and 6.59 μM). SAR analysis
-
How should Product B4186, Butyrylcholinesterase from human serum, be reconstituted?
1 answer-
Helpful?
-
-
What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
Helpful?
-
-
Are any buffer salts present in Product B4186, Butyrylcholinesterase from human serum?
1 answer-
This product was lyophilized from 20 mM ammonium bicarbonate, which is a volatile buffer. So it would be expected to contain only traces of buffer salts.
Helpful?
-
-
What amount of enzyme is present in 1 vial of Product B4186, Butyrylcholinesterase from human serum?
1 answer-
Each vial will contain at least 5 units. The vial content is given on the certificate of analysis of each lot. The vial may be substantially overpacked.
Helpful?
-
Active Filters
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service
