A7510
Adenosine 5′-Triphosphatase from porcine cerebral cortex
lyophilized powder, ≥0.3 units/mg protein, pH 7.8
Synonym(s):
ATP phosphohydrolase, ATPase
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5 UNITS
€332.00
5 X 5 UNITS
€751.00
€332.00
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5 UNITS
€332.00
5 X 5 UNITS
€751.00
About This Item
CAS Number:
MDL number:
UNSPSC Code:
12352204
NACRES:
NA.54
€332.00
Please contact Customer Service for Availability
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biological source
Porcine cerebral cortex
Quality Level
description
Sodium and Potassium-Activated
form
lyophilized powder
specific activity
≥0.3 units/mg protein, pH 7.8
composition
Protein, 5.0-25.0% biuret
technique(s)
inhibition assay: suitable
storage temp.
−20°C
Gene Information
pig ... ATP1A1(397481) , ATP1B1(396898)
Application
ATPase is used to liberate inorganic phosphorus from ATP. ATPase, from porcine cerebral cortex, has been used in inhibition assays of Na/K-ATPase activity [1].
Biochem/physiol Actions
This membrane enzyme hydrolyzes ATP to ADP and orthophosphate. The hydrolysis is coupled with the exchange of sodium and potassium ions across the plasma membrane. The enzyme is present in almost all tissues of higher organisms, but it is most abundant in the kidney where it reabsorbs sodium ions from the glomerular filtrate. Na+,K+-ATPase is affected by changes in intracellular calcium. It is also sensitive to ouabain and inhibited by vanadate, and (in vitro) by SPAI-1, a peptide isolated from porcine duodenum. It has been reported to be regulated by phosphorylation by protein kinase A (PKA).
This membrane enzyme hydrolyzes ATP to ADP and orthophosphate. The hydrolysis is coupled with the exchange of sodium and potassium ions across the plasma membrane. The enzyme is present in almost all tissues of higher organisms, but it is most abundant in the kidney where it reabsorbs sodium ions from the glomerular filtrate. Na+,K+-ATPase is affected by changes in intracellular calcium. It is also sensitive to ouabain and inhibited by vanadate, and (in vitro) by SPAI-1, a peptide isolated from porcine duodenum. It has been reported to be regulated by phosphorylation by protein kinase A (PKA). Bufalin, and other cardiotonic steroids, may inhibit Na+/K+-ATPase activity and induce the differentiation of HL60, U937, and ML1 cells [1].
Unit Definition
One unit will liberate 1.0 μmole of inorganic phosphorus from ATP per min at pH 7.8 at 37 °C in the presence of Na+, K+, and Mg2+.
Physical form
Lyophilized powder containing 90% sucrose, 0.4% EDTA Na4 and 0.06% NaCl
Preparation Note
Sodium iodide extracted fraction of Nakao, et al., Biochem. Biophys. Res. Commun., 19, 755 (1965).
inhibitor
Product No.
Description
Pricing
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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