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32857

Sigma-Aldrich

Wright Stain solution

according to Wright

Synonym(s):

Eosin Methylene blue solution according to Wright, Wright’s eosin methylene blue

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1 L
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About This Item

MDL number:
MFCD00005038
UNSPSC Code:
12171500
PubChem Substance ID:
329754363
NACRES:
MA.02

€80.10

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form

liquid

Quality Level

200

quality

according to Wright

impurities

methanol

density

0.8 g/cm3

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[K+].COC(=O)c1ccccc1C2=C3C=C(Br)C(=O)C(Br)=C3Oc4c(Br)c([O-])c(Br)cc24

InChI

1S/C21H10Br4O5.K/c1-29-21(28)9-5-3-2-4-8(9)14-10-6-12(22)17(26)15(24)19(10)30-20-11(14)7-13(23)18(27)16(20)25;/h2-7,26H,1H3;/q;+1/p-1

InChI key

NXBZXYJXNIFCRJ-UHFFFAOYSA-M

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General description

Wright′s stain solution is commercially available and is generally used in hematology. The procedure of making it includes heating methylene blue with sodium bicarbonate, followed by combining the mixture with eosin. It is an alkaline solution; hence its concentration is continuously changing, owing to the progressive oxidizing action of the alkali, therefore Giemsa′s stain is added to preserve the azurophilic properties of the polychrome dyes.[1]

Application

Wright stain solution was used during the exudate analysis of white blood cell concentration.[2]

Signal Word

Danger

Hazard Classifications

Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Eye Irrit. 2 - Flam. Liq. 2 - Skin Sens. 1 - STOT SE 1

Target Organs

Eyes,Central nervous system

Storage Class Code

3 - Flammable liquids

WGK

WGK 3

Flash Point(F)

51.8 °F

Flash Point(C)

11 °C

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

EU REACH Annex XVII (Restriction List)

CAS No.

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Certificates of Analysis (COA)

Lot/Batch Number
BCCM6752
BCCH2127
BCCG5787
BCCH9873
BCCG7552

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Biocompatibility and biofouling of MEMS drug delivery devices.
Biomaterials, 24 (11), 1959-1967 (2003)
A simplified Wright's stain technique for routine avian blood smear staining.
Shen PF and Patterson LT
Poultry Science, 62 (5), 923-924 (1983)
Qian Kong et al.
Microvascular research, 130, 104009-104009 (2020-04-26)
The purpose of the present study was to investigate the possible role of TIPE2 on acute lung injury (ALI) induced by myocardial ischemia/reperfusion (MIR) in diabetic rats. Sprague-Dawley (SD) rats were randomly separated into four groups: control+sham (C + sham); control+MIR (C + MIR);
Young-Joo Jeong et al.
Anatomy & cell biology, 43(4), 294-302 (2011-01-27)
Asthma is a Th2-dependent disease mediated by IgE and Th2 cytokines, and asthmatic patients suffer from oxidative stresses from abnormal airway inflammation. Vitamin C is a micro-nutrient functioning as an antioxidant. When administered at a mega-dose, vitamin C has been
Qian Kong et al.
Experimental and therapeutic medicine, 17(5), 3598-3606 (2019-04-17)
Acute lung injury (ALI) is a critical syndrome that is associated with high morbidity and mortality rates. The activation of the Fas/Fas ligand (FasL) signaling pathway may be an important pathophysiological mechanism during ALI development. Penehyclidine hydrochloride (PHC) has been

Questions

  1. Can this stain be used for analysis of WBC in bronchoalveolar lavage fluid? And what protocol to use for that? 

    1 answer

    1. This product is not specifically tested for use in this application. It would be up to the end user to determine suitability. Below find a link to a reference that may be helpful, as well as a link to the general protocol for this product.

      Wright Stain in BAL:
      https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4019586/

      Wright Stain Protocol
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/236/081/ws.pdf

      Helpful?

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