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M8802

Millipore

Malonate broth

pH 6.7±0.2 (25 °C), NutriSelect® Plus, suitable for microbiology

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About This Item

UNSPSC Code:
41171607
NACRES:
NA.85

sterility

non-sterile

Quality Level

200

form

powder

manufacturer/tradename

NutriSelect® Plus

technique(s)

microbe id | utilization test: suitable
microbiological culture: suitable

pH

6.7±0.2 (25 °C)

application(s)

food and beverages
water monitoring
microbiology

suitability

Klebsiella spp.
Salmonella spp.
nonselective and differential for Enterobacter spp.
nonselective and differential for Escherichia coli
nonselective and differential for coliforms

General description

Malonate broth is recommended for the differentiation of Enterobacter and Escherichia.

Application

Malonate broth has been used to evaluate the utilization of malonate by Enterobacter sakazakii. It has also been used to differentiate between Cronobacter sakazakii and Cronobacter malonaticus isolates.

Components

Components (g/L)
Ammonium sulfate, 2.00
Dipotassium phosphate, 0.60
Monopotassium phosphate, 0.40
Sodium chloride, 2.00
Sodium malonate, 3.00
Bromo thymol blue, 0.025

Preparation Note

Suspend 8 g of Malonate Broth in 1000 ml of distilled water. Dispense and sterilize by autoclaving at 15 lbs. pressure (121°C) for 15 minutes. Avoid the addition of carbon and nitrogen from other sources.

Footnote

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
BCCM3470
BCCL3002
BCCK1989
BCCK4950
BCCH1147

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Amy Strydom et al.
Food microbiology, 28(8), 1472-1477 (2011-09-20)
Members of the genus Cronobacter are opportunistic pathogens associated with life-threatening infections in immuno-compromised individuals. Polyphasic analysis has facilitated the classification of the novel genus Cronobacter containing five species. However, since this recent reclassification there are not many identification methods
Carol Iversen et al.
BMC evolutionary biology, 7, 64-64 (2007-04-19)
Enterobacter sakazakii is an opportunistic pathogen that can cause infections such as necrotizing enterocolitis, bacteraemia, meningitis and brain abscess/lesions. When the species was defined in 1980, 15 biogroups were described and it was suggested that these could represent multiple species.
Jung-Beom Kim et al.
Journal of microbiology and biotechnology, 21(5), 509-514 (2011-05-28)
This study was conducted to investigate the phenotypic and genotypic characteristics of Korean isolates of Cronobacter spp. (Enterobacter sakazakii). A total of 43 Cronobacter spp., including 5 clinical isolates, 34 food isolates, 2 environmental isolates, and 2 reference strains (C.
Ewing, W., et al.
Publ. Hlth. Lab., 15, 153-153 (1957)
MacFaddin, J
Media for Isolation-Cultivation-Identification-Maintenance of Medical Bacteria, 1 (1985)

Articles

Differentiation and Detection of Escherichia coli, Coliforms and Enterobacteriaceae

An article concerning selective growth media for differentiation and detection of Escherichia coli and other coliforms.

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