11040596001

Interferon-γ, human (hIFN-γ)

from human, 95% (SDS-PAGE), solution, >2 x 10^7 U/mg

• Synonym(s): SYBR, qPCR
• UNSPSC Code: 12352207

Properties

• biological source: human
• Quality Level: 100
• recombinant: expressed in E. coli
• description: recombinant (E. coli)
• Assay: 95% (SDS-PAGE)
• form: solution
• potency: <0.05 ng/mL EC₅₀
• specific activity: >2 x 10^7 U/mg
• mol wt: 16,700 Da
• packaging: pkg of 100,000 U (5 μg, 1 ml)
• manufacturer/tradename: Roche
• storage condition: avoid repeated freeze/thaw cycles
• impurities: <0.1 EU/μgtested (LAL test)
• UniProt accession no.: P01579
• storage temp.: −20°C
• Gene Information: human ... IFNG(3458)

Description

General description

IFN-γ is produced by T-lymphocytes stimulated by antigen or by T-cell mitogens.[1] Under nondenaturating conditions its molecular weight values range from 32,000 to 73,000 indicating that recombinant human IFN-γ exists as a dimer or higher oligomers.

Specificity

Human IFN-γ is effective on human cells, but not on mouse or rat cells.

Application

Interferon-γ, human (hIFN-γ) has been used to stimulate mesenchymal stem cells (MSCs).[2] It has also been used as a supplement Dulbecco′s modified Eagle′s medium/Ham′s medium for the cultivation of human colorectal carcinoma cell line T84 cells.[3]

Interferon-γ, human (hIFN-γ), can be used to investigate IFN-γ activities in human cell systems.[4]

Biochem/physiol Actions

A broad range of biological activities has been attributed to IFN-γ (e.g., the establishment of the antiviral state, immunoregulatory functions, antiproliferative effects and inhibition of cell growth). The anti-proliferative effects of IFN-γ are superior to those of either IFN-α or IFN-β. Growth inhibition is dependent on cell type, dose, and length of exposure. One of IFN-γ′s primary functions might be as an immunoregulatory agent: IFN-γ induces MHC antigens on many cells, Fc-receptors on monocytes and macrophages, IL-2 receptors on T-cells, enhances activity of macrophages, polymorphonuclear leukocytes, T-lymphocytes, and NK-cells (MAF), and is also involved in the regulation of B-cells.

Interferon-γ (IFN-γ) is an essential component of acquired immunity.[5] A broad range of biological activities has been attributed to IFN-γ (e.g., the establishment of the antiviral state, immunoregulatory functions,[5] antiproliferative effects and inhibition of cell growth). The anti-proliferative effects of IFN-γ [6] are superior to those of either IFN-α or IFN-β. Growth inhibition is dependent on cell type, dose, and length of exposure. One of IFN-γ′s primary functions might be as an immunoregulatory agent: IFN-γ induces MHC antigens on many cells, Fc-receptors on monocytes and macrophages, IL-2 receptors on T-cells, enhances activity of macrophages, polymorphonuclear leukocytes, T-lymphocytes, and NK-cells (MAF), and is also involved in the regulation of B-cells.

Quality

The raw material used for this preparation was tested for HBs antigen and for the presence of antibodies to HIV-1, HIV-2, and HCV found to be negative.
Endotoxin level: <0.1EU/μg (LAL-test), <10EU/ml (LAL-test)
Note: 1EU corresponds to 0.1ng

Sequence

Chain Length 143 AA

The primary structure of recombinant human IFN-γ (143 amino acids) is identical to that of natural human IFN-γ (146 amino acids), however, recombinant IFN-γ has three amino acids less and is not glycosylated. Glycosylation is not essential for biological activity.

Unit Definition

EC₅₀ definition: The amount of hIFN-γ that is required to produce equivalent antiviral activity to that expressed by 1 unit of the NIH IFN-γ reference standard (Gg 23– 901-530) (WISH cells-EMC virus/cytopathic effect) (1 unit equals ≤0.05 ng/ml).

Physical form

100,000U/ml or 1,000,000U/ml of Human IFN-γ in 0.1M phosphate buffer saline (PBS) (pH 7.0), 2.5% sucrose (w/v), and 2.5% human serum albumin (HSA) (w/v), filtered through a 0.2μm pore size membrane.

Preparation Note

Working solution: Solvent is recommended in double-distilled water.
Storage conditions (working solution): Stability in culture: Activity is present for at least 18 hours in culture medium.

Other Notes

EC ₅₀ : <0.05ng/ml (hIFN-γ , NIH, reference standard, Gg 23–901-530)

For life science research only. Not for use in diagnostic procedures.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): No data available
• Flash Point(C): No data available