The concentration provided at 200uM in DMSO solution, final volume 50ul. Please spin down this material before usage to be able to collect all the solution in the bottom of the tube. It is recommended to use the material at 1:1000 dilution, but this is sample dependent.
SCT083
BioTracker™ MemBright 488 Live Cell Dye
Synonym(s):
Live cell imaging probe, Live cell membrane probe, green
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1 VIAL
€183.00
€183.00
Estimated to ship onMarch 10, 2025Details
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1 VIAL
€183.00
About This Item
UNSPSC Code:
41106514
NACRES:
NA.47
€183.00
Estimated to ship onMarch 10, 2025Details
Recommended Products
mol wt
observed mol wt 1218.5 kDa
Quality Level
packaging
vial of 50 μL
manufacturer/tradename
Millipore
growth mode
N/A
technique(s)
cell based assay: suitable
flow cytometry: suitable
detection method
fluorometric
shipped in
dry ice
storage temp.
−20°C
General description
The BioTracker™ Membright-488 live cell dye is a “turn-on” fluorescence probe emitting in the far red spectrum for the imaging of plasma membranes of live cells.
Specific plasma membrane markers are essential for delineating the cell. Membright dyes exploit a unique fluorogenic mechanism, existing in a self-quenched nanoparticle state. Fluorescence is turned on upon contact with and propagation within the plasma membrane, producing bright, background-free signal. The Membright live cell membrane dyes are compatible with two-photon imaging, superresolution imaging, and fixed cell preparations. The high photostability and lack of cytotoxicity of Membright dyes make them suitable for long-term live cell imaging. The suite of superior characteristics of Membright plasma membrane dyes permit use in a wide spectrum of fluorescence microscopy applications and flow cytometry.
Spectral Properties
Fluorescence images obtained by λex = 488 nm and emission range of 500-550 nm.
Reference
Collot M et al. MemBright: A family of fluorescent membrane probes for advanced cellular imaging and neuroscience. Cell Chem. Biol. 2019, 26:600-614
Specific plasma membrane markers are essential for delineating the cell. Membright dyes exploit a unique fluorogenic mechanism, existing in a self-quenched nanoparticle state. Fluorescence is turned on upon contact with and propagation within the plasma membrane, producing bright, background-free signal. The Membright live cell membrane dyes are compatible with two-photon imaging, superresolution imaging, and fixed cell preparations. The high photostability and lack of cytotoxicity of Membright dyes make them suitable for long-term live cell imaging. The suite of superior characteristics of Membright plasma membrane dyes permit use in a wide spectrum of fluorescence microscopy applications and flow cytometry.
Spectral Properties
Fluorescence images obtained by λex = 488 nm and emission range of 500-550 nm.
Reference
Collot M et al. MemBright: A family of fluorescent membrane probes for advanced cellular imaging and neuroscience. Cell Chem. Biol. 2019, 26:600-614
Application
Purity: > 98% confirmed by HPLC, HNMR, LC-MS and elemental analysis.
Features and Benefits
Green fluorescent probe for efficient, selective labeling of the plasma membrane in live cells. Suitable for superresolution microscopy.
Physical form
Green solution
Storage and Stability
Store long term at -20°C, protected from light.
Other Notes
Live cell fluorescent imaging, flow cytometry
Legal Information
BioTracker is a trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Flash Point(F)
188.6 °F - closed cup
Flash Point(C)
87 °C - closed cup
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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What is the concentration of membright 488. Only info I have is that it is 50 microlitres. Publications use it at 20 nM
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