Skip to Content
Merck
All Photos(2)

Key Documents

View All Documentation

SCM142

Sigma-Aldrich

PLTMax Human Platelet Lysate

PLTMax Human Platelet Lysate is a growth factor rich supplement that is a superior alternative to fetal bovine serum (FBS) for human mesenchymal stem cell (MSC) culture. 500ml.

Synonym(s):

PLTMax hPL, hPL

Sign Into View Organizational & Contract Pricing

Select a Size

500 ML
€1,670.00

€1,670.00

Please contact Customer Service for Availability
All Photos(2)

Select a Size

Change View
500 ML
€1,670.00

About This Item

UNSPSC Code:
41106514
eCl@ss:
32011203
NACRES:
NA.71

€1,670.00

Please contact Customer Service for Availability

biological source

human

Quality Level

100

form

liquid

manufacturer/tradename

Chemicon®

technique(s)

cell culture | stem cell: suitable

input

sample type mesenchymal stem cell(s)

shipped in

ambient

Related Categories

General description

PLTMax Human Platelet Lysate is a growth factor rich supplement that is a superior alternative to fetal bovine serum (FBS) for human mesenchymal stem cell (MSC) culture. Optimal growth of MSCs can be achieved with 5% PLTMax Human Platelet Lysate. PLTMax Human Platelet Lysate has been used to effectively to grow mesenchymal stem cells derived from other species including mouse, rabbit and porcine.



PLTMax Human Platelet Lysate is derived from normal human donor platelets collected at U.S. blood centers. Multiple donors units are pooled in large batch sizes and manufactured to produce a consistent product. Heparin (porcine) approved for human intravenous use is used in the preparation of PLTMax Human Platelet Lysate. This product contains human derived materials and must be treated as potentially infectious. Universal precautions for biological samples should be used in handling and disposal.

Application

Instructions for Use
• Add Heparin to the media at a final concentration of 2 U/mL. Failure to add heparin may result in coagulation of certain media.
• For optimal growth of human mesenchymal stem cells, add 5% vol/vol PLTMax Human Platelet Lysate to usual cell culture medium such as DMEM and αMEM.
• Plate cells at approximately 1.5x10^3 cells per cm2.
• Do not allow primary MSC confluence to exceed 80%.

Particulate Formation
• Product has been aseptically filtered with 0.2 µm filters. Particulates will be observed after thawing. Particulate formation is normal will not affect performance even in the presence of large particulates. Filtration is not recommended.
• To reduce particulates from forming, minimize freeze/thaw cycles and extended storage at 4°C. Some particulate formation can be reversed with brief incubation at 37°C, if desired.

Quality

PLTMax Human Platelet Lysate is manufactured with the highest quality of raw materials. All human derived materials are obtained from government inspected facilities and are of US origin. Each donor unit was approved for human use and has been tested for infectous diseases including HIV-1, HIV-2, HIV-NAT, HCV, HBsAg, and RPR for Syphilis.

Each lot is extensively tested for the following parameters:
pH: 6.8-7.8
Total protein: 4.0-6.5 g/dl
Endotoxin USP <8>: <10 EU/mL
Mycoplasma: Not detected
Sterility Testing: Negative for growth

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Articles

PLTMax® Human Platelet Lysate (hPL): A Superior Serum-Free Media Supplement for Robust Mesenchymal Stem Cell Expansion

PLTMax® Human Platelet Lysate (hPL) is a superior serum-free and xeno-free media supplement alternative to fetal bovine serum (FBS) for human mesenchymal stem cell (MSC) cultures.

Mesenchymal Stem Cell FAQs

Frequently asked questions about mesenchymal stem cells including MSC derivation, expansion, differentiation and allogenic stem cell therapy.

Questions

  1. PLTMaxを使用して実験をしているのですが、PRPとの性質の違いはありますか。一般的に使用されているPRPとの違いをご教示ください。（製法や、ヘパリン以外の添加物などあれば教えていただきたいです。）

    1 answer

    1. The main difference is that PRP contains "whole" platelets (a type of blood cell), while PLTMax is a platelet "Lysate" i.e., it is a platelet "extract" that contains platelet growth factors rather than whole platelet cells.

      The manufacturing process for SCM142 is summarized below. An appropriate lysis buffer is used to lyse the platelets to prepare the lysate/extract. Further details are proprietary.

      Please see the details below:

      1. PRP stands for Platelet-rich plasma.
      "Platelet-rich plasma consists of two elements: plasma, or the liquid portion of blood, and platelets, a type of blood cell that plays an important role in healing throughout the body. Platelets are well-known for their clotting abilities, but they also contain growth factors that can trigger cell reproduction and stimulate tissue regeneration or healing in the treated area. Platelet-rich plasma is simply blood that contains more platelets than normal.

      To create platelet-rich plasma, clinicians take a blood sample from the patient and place it into a device called a centrifuge that rapidly spins the sample, separating the other components of the blood from the platelets and concentrating them within the plasma".

      This information can be accessed in the link below:

      https://www.hopkinsmedicine.org/health/treatment-tests-and-therapies/plateletrich-plasma-prp-treatment#:~:text=Platelet%2Drich%20plasma%20(PRP)%20injections%20are%20gaining%20popularity%20for,healing%20in%20a%20specific%20area.

      2. The following information below is found for Product SCM142 on the Product Detail Page:

      PLTMax® Human Platelet Lysate is a growth factor-rich supplement that is a superior alternative to fetal bovine serum (FBS) for human mesenchymal stem cell (MSC) culture. Optimal growth of MSCs can be achieved with 5% PLTMax Human Platelet Lysate. PLTMax Human Platelet Lysate has been used to effectively grow mesenchymal stem cells derived from other species, including mice, rabbits, and porcine.

      PLTMax Human Platelet Lysate is derived from normal human donor platelets collected at U.S. blood centers. Multiple donor units are pooled in large batch sizes and manufactured to produce a consistent product. Heparin (porcine), approved for human intravenous use, is used to prepare PLTMax Human Platelet Lysate. This product contains human-derived materials and must be treated as potentially infectious. Universal precautions for biological samples should be used in handling and disposal.

      Helpful?

Reviews

No rating value

Active Filters

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service