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MABT850M

Sigma-Aldrich

Anti-Podoplanin Antibody, clone PMab-2

clone PMab-2, from mouse

Synonym(s):

E11 antigen epitope, Glycoprotein 38, Gp38, OTS-8, RTI140, T1-alpha, T1A, Type I cell 40 kDa protein

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.43

biological source

mouse

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

PMab-2, monoclonal

species reactivity

rat

packaging

antibody small pack of 25 μg

technique(s)

ELISA: suitable
flow cytometry: suitable
immunohistochemistry: suitable (paraffin)
western blot: suitable

isotype

IgG1κ

UniProt accession no.

target post-translational modification

unmodified

General description

Podoplanin (UniProt: Q64294; also known as E11 antigen epitope, Glycoprotein 38, Gp38, OTS-8, RTI140, T1-alpha, T1A, Type I cell 40 kDa protein) is encoded by the Pdpn (also known as Gp38, Ots8) gene (Gene ID: 54320) in rat. Podoplanin serves as the endogenous ligand of C-type lectin-like receptor-2 (CLEC-2) and is highly expressed in various tumors and in some normal cells. It may be involved in cell migration and/or actin cytoskeleton organization. Podoplanin is localized to actin-rich microvilli and plasma membrane projections, such as filopodia, lamellipodia, and ruffles. It is synthesized with a signal peptide of 22 amino acids, which is cleaved to produce the mature form. Podoplanin contains an extracellular domain (aa 23-135), a helical domain (aa 136-156) and a cytoplasmic region (aa 157-166). Podoplanin is extensively O-glycosylated and contains sialic acid residues. O-glycosylation is believed to be essential for its platelet aggregation activity. Clone PMab2 exhibits high affinity for podoplanin (Kd = 0.59 nM). (Ref.: Oki, H et al. (2015). Monoclon. Antib. Immunodiagn. Immunother. 34(6); 396-403).

Specificity

Clone PMab-2 detects podoplanin in rat spleen and kidney. It targets an epitope within 14 amino acids from the N-terminal region.

Immunogen

KLH-conjugated linear peptide corresponding to 14 amino acids from the N-terminal region of rat Podopalnin.

Application

Anti-Podoplanin, clone PMab-2, Cat. No. MABT850, is a mouse monoclonal that detects rat podoplanin and has been tested for use in ELISA, Flow Cytometry, Immunohistochemistry (Paraffin), and Western Blotting.
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Podoplanin in rat spleen tissue.

Flow Cytometry Analysis: A representative lot detected Podoplanin in Flow Cytometry applications (Oki, H., et. al. (2015). Monoclon Antib Immunodiagn Immunother. 34(6):396-403).

Western Blotting Analysis: A representative lot detected Podoplanin in Western Blotting applications (Oki, H., et. al. (2015). Monoclon Antib Immunodiagn Immunother. 34(6):396-403).

Immunohistochemistry Analysis: A representative lot detected Podoplanin in Immunohistochemistry applications (Oki, H., et. al. (2015). Monoclon Antib Immunodiagn Immunother. 34(6):396-403).

ELISA Analysis: A representative lot detected Podoplanin in ELISA applications (Oki, H., et. al. (2015). Monoclon Antib Immunodiagn Immunother. 34(6):396-403).

Quality

Evaluated by Immunohistochemistry in rat kidney tissue sections.

Immunohistochemistry Analysis: A 1:250 dilution of this antibody detected Podoplanin in rat kidney tissue sections.

Target description

17.58 kDa calculated.

Physical form

Format: Purified

Other Notes

Concentration: Please refer to lot specific datasheet.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1


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