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Key Documents

MABE649

Sigma-Aldrich

Anti-Histone H3.3 Antibody, clone RM190

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

antibody form

purified antibody

Quality Level

clone

RM190
monoclonal

purified by

using Protein A

species reactivity

human, rabbit

concentration

(Please refer to lot specific datasheet.)

technique(s)

multiplexing: suitable
western blot: suitable

isotype

IgG

UniProt accession no.

General description

A peptide corresponding to human Histone H3.3.

Specificity

This antibody reacts to Histone H3.3, independent of post-translational modifications. No cross reactivity with Histone H3.1 or other histone proteins.

Application

Multiplex Analysis (Mplex): 0.05-0.5 μg/mL from a representative lot was used in Multiplex.

Quality

Evaluated by Western Blotting in HeLa whole cell lysate, recombinant Histone H3.3 and recombinant Histone H3.1.

Western Blotting Analysis (WB): 1 μg/mL of this antibody detected Histone H3.3 in HeLa whole cell lysate and recombinant Histone H3.3.

Physical properties

~17 kDa observed. Uncharacterized bands may be observed in some lysate(s).

Physical form

Rabbit monoclonal in PBS with 1% BSA and 0.09% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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