CHR103
Digitonin solution
high purity
Synonym(s):
Digitonin Detergent, digitin solution
Sign Into View Organizational & Contract Pricing
All Photos(1)
About This Item
UNSPSC Code:
12161503
eCl@ss:
32161000
Pricing and availability is not currently available.
Recommended Products
General description
Digitonin is a non-ionic steroid saponin derived from Digitalis purpurea. Historically, digitonin has been used to solubilize receptors and permeabilize membranes for sample preparation and analysis. Digitonin interacts with cholesterol creating a digitonin-cholesterol complex, which leads to subsequent removal of cholesterol and cellular membrane permeabilization. Therefore, cellular compartments composed of low amounts of cholesterol are protected from digitonin effects, particularly the nuclear membrane.
Due to digitonin′s mechanism of action, many other epigenetic applications utilize digitonin for nuclear extraction. For example, assay for Transposase-Accessible Chromatin with high throughput sequencing (ATAC-Seq) is a valuable method to map open chromatin sites and assess the epigenetic landscape. In combination with other detergents, a low concentration of digitonin is employed to isolate the nuclear pellet followed by digestion of the nuclei with an increased concentration of digitonin5. In our ChIC (Chromatin Immuno-Cleavage) Kits (part #s: CHR100-102), digitonin is utilized for antibody targeting of micrococcal nuclease for chromatin tagging.
Due to digitonin′s mechanism of action, many other epigenetic applications utilize digitonin for nuclear extraction. For example, assay for Transposase-Accessible Chromatin with high throughput sequencing (ATAC-Seq) is a valuable method to map open chromatin sites and assess the epigenetic landscape. In combination with other detergents, a low concentration of digitonin is employed to isolate the nuclear pellet followed by digestion of the nuclei with an increased concentration of digitonin5. In our ChIC (Chromatin Immuno-Cleavage) Kits (part #s: CHR100-102), digitonin is utilized for antibody targeting of micrococcal nuclease for chromatin tagging.
Other Notes
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service
