Skip to Content
Merck
All Photos(1)

Key Documents

AP156P

Sigma-Aldrich

Goat Anti-Rabbit IgG Antibody, Fc, HRP conjugate

0.8 mg/mL, Chemicon®

Synonym(s):

Goat anti-Rabbit IgG

Sign Into View Organizational & Contract Pricing


About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.46

biological source

goat

Quality Level

conjugate

peroxidase conjugate

antibody form

F(ab′)2 fragment of affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

species reactivity

rabbit

manufacturer/tradename

Chemicon®

concentration

0.8 mg/mL

technique(s)

ELISA: suitable
western blot: suitable

shipped in

wet ice

target post-translational modification

unmodified

Related Categories

Specificity

Based on immunoelectrophoresis, the antibody reacts with the heavy chains on rabbit IgG but not with the light chains on most rabbit immunoglobulins. No antibody was detected against rabbit IgM, or against immunoglobulin serum proteins, but antibodies may cross-react with immunoglobulins from other species.

Application

ELISA and Western Blotting 1:5,000 - 1:100,000

Immunohistochemistry 1:500 - 1:5,000

Optimal working dilutions must be determined by the end user.
Research Category
Secondary & Control Antibodies
Research Sub Category
Fragment Specific Secondary Antibodies
This Goat anti-Rabbit IgG Antibody, Fc, HRP conjugate is validated for use in ELISA, WB for the detection of Rabbit IgG.

Physical form

Liquid. Buffer =0.01M Sodium Phosphate, 0.25 M NaCl, pH 7.6, and mixed with 15mg/mL BSA as stabilizer containing no preservatives.

RECONSTITUTION:

Reconstitute in 2mL deionized water.

Storage and Stability

Maintain lyophilized product at 2-8°C for up to 12 months. After reconstitution the product is stable for several weeks at 2-8°C as an undiluted liquid. Use sterile technique as formulation has no preservatives. For extended storage after reconstitution, we suggest adding an equal volume of glycerol to make a final concentration of 50% glycerol followed by storage at -20°C, with or without aliquoting for up to 12 months. Please note the concentration of protein (and buffer salts) will decrease to one-half of the original after the addition of glycerol. Avoid repeated freeze/thaw cycles.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Not finding the right product?  

Try our Product Selector Tool.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Kimie Date et al.
FEBS open bio, 9(4), 755-768 (2019-04-16)
Liver cirrhosis (LC) is a disease characterized by pathological accumulation and alteration of extracellular matrix (ECM) proteins; the interaction between two such proteins, collagen and vitronectin (VN), is considered to be the key to controlling ECM remodeling in liver cirrhosis.
Kimie Date et al.
Scientific reports, 11(1), 12023-12023 (2021-06-10)
Vitronectin (VN) is a glycoprotein found in extracellular matrix and blood. Collagen, a major extracellular matrix component in mammals, is degraded by cathepsin K (CatK), which is essential for bone resorption under acidic conditions. The relationship between VN and cathepsins
Human cytomegalovirus major immediate early 1 protein targets host chromosomes by docking to the acidic pocket on the nucleosome surface.
Mucke, K; Paulus, C; Bernhardt, K; Gerrer, K; Schon, K; Fink, A; Sauer et al.
Journal of virology null
Hai-Ping Zhu et al.
Molecular medicine reports, 21(4), 1872-1880 (2019-09-24)
Lipopolysaccharide (LPS) induces stress inflammation and apoptosis. Pulmonary epithelial cell apoptosis, which accelerates the progression of acute lung injury (ALI)/acute respiratory distress syndrome (ARDS), is the leading cause of mortality in patients with ALI/ARDS. The nephroblastoma overexpressed protein (CCN3), an
Morten M Callesen et al.
Molecular oncology, 11(11), 1616-1629 (2017-09-08)
Transgenic porcine cancer models bring novel possibilities for research. Their physical similarities with humans enable the use of surgical procedures and treatment approaches used for patients, which facilitates clinical translation. Here, we aimed to develop an inducible oncopig model of

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service