Skip to Content
Merck
All Photos(1)

Key Documents

View All Documentation

ABT13

Sigma-Aldrich

Anti-Sarcolipin Antibody

from rabbit, purified by affinity chromatography

Synonym(s):

Sarcopolin

Sign Into View Organizational & Contract Pricing

Select a Size

100 μG
€474.00

€474.00

Please contact Customer Service for Availability
Request a Bulk Order
All Photos(1)

Select a Size

Change View
100 μG
€474.00

About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

€474.00

Please contact Customer Service for Availability
Request a Bulk Order

biological source

rabbit

Quality Level

100

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

rabbit

species reactivity (predicted by homology)

rat (based on 100% sequence homology), human (immunogen homology), mouse (based on 100% sequence homology)

technique(s)

western blot: suitable

NCBI accession no.

NP_003054

UniProt accession no.

O00631

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... SLN(6588)

General description

Sarcolipin (SLN) is a multidomain protein containing lumenal, cytoplasmic, and transmembrane domains. It is found in the membrane of the sarcoplasmic/endoplasmic reticulum of cardiac cells, and is involved in maintaining calcium homestasis underlying muscle contractility in the heart. Sarcoplipin achieves these effects through its regulation of the sarco(endo)plasmic reticulum Ca2+ ATPase (SERCA2a), a calcium transport protein that actively pumps calcium from the cytoplasm into the sarcoplasmic reticulum, resulting in relaxation of the heart muscles. Sarcolipin may also interact with phospholamban, and may contribute to heart failure.

Immunogen

KLH-conjugated linear peptide corresponding to the C-terminus of human Sarcolipin.

Application

Detect Sarcolipin using this Anti-Sarcolipin Antibody validated for use in Western Blotting.
Research Category
Cell Structure
Research Sub Category
Cytoskeleton

Quality

Evaluated by Western Blot in rabbit quadraceps tissue lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected Sacolipin in rabbit quadraceps tissue lysate.

Target description

~6 kDa observed. An uncharacterized band may be observed at ~13 kDa in some tissue lysates.

Physical form

Affinity purified
Purified rabbit polyclonal serum in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Rabbit quadraceps tissue

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Not finding the right product?  

Try our Product Selector Tool.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

John Butler et al.
Archives of biochemistry and biophysics, 569, 26-31 (2015-02-11)
Studies in sarcolipin knockout mice have led to the suggestion that skeletal muscle sarcolipin plays a role in thermogenesis. The mechanism proposed is uncoupling of the sarcoplasmic reticulum calcium pump. However, in other work sarcolipin was not detected in mouse
Junaid A B Zaman et al.
International journal of cardiology, 220, 580-588 (2016-07-09)
Post-operative atrial fibrillation (POAF) is a major health economic burden. However, the precise mechanisms in POAF remain unclear. In other forms of AF, sites of high dominant frequency (DF) in sinus rhythm (SR) may harbour 'AF nests'. We studied AF
Victoria L Wyckelsma et al.
American journal of human genetics, 108(3), 446-457 (2021-02-19)
The protein α-actinin-3 expressed in fast-twitch skeletal muscle fiber is absent in 1.5 billion people worldwide due to homozygosity for a nonsense polymorphism in ACTN3 (R577X). The prevalence of the 577X allele increased as modern humans moved to colder climates
Joseph M Autry et al.
Veterinary sciences, 7(4) (2020-11-19)
Ca2+ regulation in equine muscle is important for horse performance, yet little is known about this species-specific regulation. We reported recently that horse encode unique gene and protein sequences for the sarcoplasmic reticulum (SR) Ca2+-transporting ATPase (SERCA) and the regulatory
Diane M Sepa-Kishi et al.
Scientific reports, 7(1), 15430-15430 (2017-11-15)
This study investigated fiber type-specific metabolic responses and the molecular mechanisms that regulate glucose and fat metabolism in oxidative and glycolytic muscles upon cold acclimation. Male Wistar rats were exposed to cold (4 °C) for 7 days, and then glycogen synthesis
View All Related Papers

Questions

  1. Sarcolipin soll bei Broken Heart Cardiomyopathy (Takotsubo-CM)in linksventrikulären stark Herzmuskelzellen erhöht sein; da ich soeben einen TAKOTSUBO-Fall obduziert habe, möchte ich diesa testen.. Testprobe des Ak und ggf Literatur verfügbar?

    1 answer

    1. Product ABT3, Anti-Sarcolipin Antibody is suitable for Western Blot applications and has not been tested in other applications such as immunohistochemistry. You may find it helpful to view the "Peer Reviewed Papers" section on the website for product ABT13.

      We do not routinely provide samples of our antibodies, however, you may submit request for a quotation using this link: https://www.sigmaaldrich.com/support/customer-support.

      Helpful?

Reviews

No rating value

Active Filters

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service