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71296

Millipore

PhosphoSafe Extraction Reagent

Novagen′s PhosphoSafe Extraction Buffer efficiently extracts cytosolic proteins from mammalian and insect cells while preserving their phosphorylation state.

Synonym(s):

Protein Extraction Reagent

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25 ML
€155.00
125 ML
€602.00

€155.00

Please contact Customer Service for Availability
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25 ML
€155.00
125 ML
€602.00

About This Item

UNSPSC Code:
41106510
NACRES:
NA.77

€155.00

Please contact Customer Service for Availability

Quality Level

100

form

liquid

manufacturer/tradename

Novagen®

storage condition

OK to freeze

technique(s)

immunoblotting: suitable

application(s)

sample preparation

shipped in

dry ice

storage temp.

−70°C

Related Categories

General description





PhosphoSafe Extraction Buffer efficiently extracts cytosolic proteins from mammalian and insect cells while preserving their phosphorylation state. This reagent contains the same formula as CytoBuster Protein Extraction Reagent (Trim 2001), but also includes four phosphatase inhibitors: sodium fluoride, sodium vanadate, β-glycerophosphate, and sodium pyrophosphate. PhosphoSafe Extraction Reagent is compatible with kinase assays, protein interaction analysis, and other applications. This reagent is provided in convenient 25-ml bottles (1 bottle for 71296-3, 5 bottles for 71296-4).

Features and Benefits

  • Phosphorylation state preserved during extraction
  • Compatible with kinase assays

Warning

Toxicity: Toxic (F)

Other Notes

Trim, J.E. and Sawyer, D.L. 2001. inNovations12, 5.

Legal Information

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Adrienne R Gibson et al.
Cancer research, 80(7), 1401-1413 (2020-02-12)
Pharmacologic ascorbate treatment (P-AscH-, high-dose, intravenous vitamin C) results in a transient short-term increase in the flux of hydrogen peroxide that is preferentially cytotoxic to cancer cells versus normal cells. This study examines whether an increase in hydrogen peroxide is

Questions

1–2 of 2 Questions  

  1. what is recomended volume for 50mg tissue extraction? would you recomend adding protease ihnibitor too? could you share protocol for protein extraction from liver using this phosphosafe extraction reagent?

    1 answer

    1. A protocol for liver tissue particularly is not offered. Here is a protocol for tissue in general:

      Tissue homogenates are prepared by homogenizing tissue in ice-cold PhosphoSafe extraction buffer using 70 ul buffer/mg tissue. Homogenates are incubated at room temperature for 5 mins, sonicated on ice, and then subjected to centrifugation (spun at 16,000 X g, 5 mins, at 4 Celsius). The resulting supernatant is collected (the end-user will want to keep this, and can discard the insoluble pellet). Finally, protein concentration is determined by a protein assay (e.g., Bradford assay).

      To minimize proteolysis, extraction can be performed on ice, and protease inhibitors can be added.
      Please see the link below to access the full product user guide:
      https://www.emdmillipore.com/product/PhosphoSafe-Extraction-Reagent,EMD_BIO-71296#anchor_USP

      Helpful?

  2. I am doing protein isolation from the organic phase remaining after RNA isolation using Trizol/Chloroform. How can I use PhosphoSafe™ Extraction Reagent in this case?

    1 answer

    1. Phosphosafe has been tested for use in aqueous formats such as cell culture supernatants or lysates. The performance of the product in an organic phase following fractionation with Trizol has not been determined. To discuss further, we kindly ask you to navigate to the link https://www.sigmaaldrich.com/techservice, click on "Product Technical Inquires" under the Products Section with all the required information so that a member of our team can reach out to you to assist further. Thank you.

      Helpful?

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