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biological source
rabbit
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
polyclonal
form
liquid
contains
≤0.1% sodium azide as preservative
species reactivity
mouse, human
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
avoid repeated freeze/thaw cycles
isotype
IgG
storage temp.
−20°C
target post-translational modification
unmodified
General description
Anti-Catalase, rabbit polyclonal, recognizes the ~60-65 kDa human and mouse catalase. It is validated for ELISA, Western blotting, and immunoelectrophoresis.
Rabbit polyclonal antibody purified by ammonium sulfate precipitation and DEAE-cellulose ion exchange chromatography. Recognizes the ~60-65 kDa (monomer MW) catalase protein under reducing conditions.
Recognizes the ~60-65 kDa catalase protein.
Immunogen
purified, human erythrocytes catalase
Application
Indirect ELISA (1:128)
Immunoblotting (1:8000; see application references)
Immunoelectrophoresis (see comments)
Paraffin Sections (see application references)
Immunoblotting (1:8000; see application references)
Immunoelectrophoresis (see comments)
Paraffin Sections (see application references)
Warning
Toxicity: Standard Handling (A)
Analysis Note
Positive Control
Red blood cells
Red blood cells
Other Notes
The 256 kDa catalase protein is a tetramer of 4 identical ~60-65 kDa protein. Exhibits a single arc by immunoelectrophoresis (IEP) vs. human red cell lysate. Variables associated with assay conditions will dictate the proper working dilution.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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Storage Class Code
12 - Non Combustible Liquids
WGK
nwg
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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