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203782

Sigma-Aldrich

5-Bromo-4-chloro-3-indolyl-β-D-galactopyranoside

A chromogenic substrate for β-galactosidase. Used to distinguish between recombinant and non-recombinant plasmids carrying β-galactosidase gene (e.g. pUR222).

Synonym(s):

5-Bromo-4-chloro-3-indolyl-β-D-galactopyranoside, X-Gal

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100 MG
€69.60
250 MG
€143.00
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€273.00
5 G
€799.00

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100 MG
€69.60
250 MG
€143.00
1 G
€273.00
5 G
€799.00

About This Item

Empirical Formula (Hill Notation):
C14H15BrClNO6
CAS Number:
Molecular Weight:
408.63
MDL number:
UNSPSC Code:
12352200
NACRES:
NA.77

€69.60


Please contact Customer Service for Availability

Request a Bulk Order

Quality Level

Assay

≥99% (HPLC)

form

solid

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
desiccated
protect from light

color

white

solubility

DMSO: 2% (20mg/mL)

shipped in

ambient

storage temp.

−20°C

SMILES string

[Cu+2].[Cu+].N3(CCN(CC3)C)Cc1c(c(cc(c1)C#N)CN2CCN(CC2)C)O.OC(O)C.OC(O)C

InChI

1S/C19H29N5O.2C2H6O2.2Cu/c1-21-3-7-23(8-4-21)14-17-11-16(13-20)12-18(19(17)25)15-24-9-5-22(2)6-10-24;2*1-2(3)4;;/h11-12,25H,3-10,14-15H2,1-2H3;2*2-4H,1H3;;/q;;;+1;+2

InChI key

GGILAJWFDJWIQO-UHFFFAOYSA-N

General description

A chromogenic substrate for β-galactosidase. Used to distinguish between recombinant and non-recombinant plasmids carrying β-galactosidase gene (e.g. pUR222).

Warning

Toxicity: Standard Handling (A)

Other Notes

Engler, K.H., et al. 1999. J. Microbiol. Methods35, 207.
Arakawa, H., et al. 1991. Anal. Biochem.199, 238.
Horwitz, J., et al. 1964. J. Med. Chem.7, 574.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Questions

  1. Hi. I would like to know for how long I can store X-Gal (-20°C) before its degradation. Thanks

    1 answer
    1. This product is assigned a product retest date of approximately 2.5 years from the date of Quality release and is considered to be a stable compound. Products that, while considered stable, may be subject to degradation over time are assigned a periodic retest date to confirm quality.  The lot specific retest date is reported on the Certificate of Analysis. 

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