Unfortunately, we do not offer guards with a 3.5µm particle size. The closest option I would recommend is 1.50434.000. It is 5 μm particle size but it will have no impact on the results because of the very small column volume.
1.50441
SeQuant® ZIC®-HILIC 100Å (3.5 µm) HPLC Columns
L × I.D. 100 mm x 2.1 mm HPLC Column, PEEK-lined stainless steel
Synonym(s):
Zwitterionic Hydrophilic Interaction Liquid Chromatography HPLC Column
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1 UNIT
€1,150.00
€1,150.00
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1 UNIT
€1,150.00
About This Item
UNSPSC Code:
41115709
€1,150.00
Please contact Customer Service for Availability
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Product Name
SeQuant® ZIC-HILIC™ 3.5µm,100Å 100 x 2.1 mm, PEEK-lined stainless steel column, suitable for HPLC
material
PEEK-lined stainless steel column
Quality Level
Agency
suitable for USP L114
product line
SeQuant®
feature
endcapped: no
parameter
550 bar pressure
70 °C max. temp.
technique(s)
HPLC: suitable
LC/MS: suitable
UHPLC-MS: suitable
UHPLC: suitable
mass spectrometry (MS): suitable (High)
column L × I.D.
10 cm × 2.1 mm
matrix
fully porous particle
matrix active group
ZIC-HILIC bonding phase
particle size
3.5 μm
pore size
100 Å pore size
operating pH
2-7.5
separation technique
hydrophilic interaction (HILIC)
storage temp.
no temp limit
General description
SeQuant® ZIC®-HILIC Columns are designed to retain and separate all types of polar and hydrophilic compounds and for robust chromatography with high selectivity and reproducibility. The silica-based ZIC®-HILIC sorbent has a bonded stationary phase consisting of a highly polar, permanent zwitterion. Separation selectivity is favored by the 1:1 zwitterion charge balance, which makes the ZIC®-HILIC Column overall neutral, with weak, but important, ionic interactions. Tuning of the selectivity on the ZIC®-HILIC Column during method development is facilitated by the pH-inpendent permanent zwitterion, ensuring that only the analytes (and not the column) are affected during eluent optimization.The Pore size of 100 Å makes this column perfectly suitable for the separation of small polar molecules under HILIC conditions.The column dimension of 250 mm length x 2.1 mm ID is the optimal choice for the separation of complex samples using a UHPLC or optimized HPLC Instrument providing low dwell volumes.Thanks to their favorable retention and robust performance, SeQuant® HILIC columns have become extremely popular in numerous laboratories around the world. For example, US FDA (United States Food and Drug Administration) has recommended SeQuant® ZIC®-HILIC for analysis of melamine and related substances. SeQuant® HILIC columns excel in stability and low bleed. These features make them particularly suitable for LC-MS applications where high background can lead to signal suppression and interference with quantitative measurements while also increasing instrument wear. Their high stability makes these high-performance HILIC columns also attractive for traditional HPLC applications.Learn more about the Supelco HPLC Column portfolio and Method development:Brochure: HPLC and UHPLC Column Selection Guide Brochure: A Practical Guide to HPLC - Method Development Guide
Legal Information
SeQuant is a registered trademark of Merck KGaA, Darmstadt, Germany
ZIC is a registered trademark of Merck KGaA, Darmstadt, Germany
ZIC-HILIC is a trademark of Merck KGaA, Darmstadt, Germany
related product
Product No.
Description
Pricing
Storage Class Code
10-13 - German Storage Class 10 to 13
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What is the recommended guard column for the Sequant ZIC Hilic column 100x2.1 mm, 3.5 um particle size, 100A pore size, with part number 1.50441.000?
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