Novel biocatalyst for aldol condensation, especially for the re-facial addition of aliphatic ketones to aldehydes. Antibody 84G3 catalyzes aldol, crossed aldol, retroaldol, decarboxylation of β-keto acids, self aldol, and Robinson annulations.
Angewandte Chemie (International ed. in English), 38(24), 3738-3741 (2000-01-29)
Nine efficient aldolase antibodies were generated by using hapten 1. This hapten unites reactive immunization and the transition state analogue approach in a single molecule. Characterization of two of these antibodies reveals that they are highly proficient (up to 1000-fold
[formula: see text] Three monoclonal aldolase antibodies, generated against a beta-diketone hapten by reactive immunization, catalyzed rapid and highly enantioselective retro-aldol reactions of ent-8a-k, providing optically pure 8a-k by kinetic resolution. Compounds (+/-)-8a, (+/-)-8g, and (+/-)-8k have been resolved in
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