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294381

Sigma-Aldrich

N,N′-(1,2-Dihydroxyethylene)bisacrylamide

97%

Synonym(s):

N,N′-Bisacryloyl-1,2-dihydroxy-1,2-ethylenediamine, DHEBA

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5 G
€89.00
25 G
€426.00

€89.00

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5 G
€89.00
25 G
€426.00

About This Item

Linear Formula:
[H2C=CHCONHCH(OH)-]2
CAS Number:
868-63-3
Molecular Weight:
200.19
Beilstein:
1941875
EC Number:
212-780-1
MDL number:
MFCD00008624
UNSPSC Code:
12162002
PubChem Substance ID:
24857712
NACRES:
NA.23

€89.00

Please contact Customer Service for Availability
Request a Bulk Order

Quality Level

200

Assay

97%

form

solid

mp

156 °C (dec.) (lit.)

SMILES string

OC(NC(=O)C=C)C(O)NC(=O)C=C

InChI

1S/C8H12N2O4/c1-3-5(11)9-7(13)8(14)10-6(12)4-2/h3-4,7-8,13-14H,1-2H2,(H,9,11)(H,10,12)

InChI key

ZMLXKXHICXTSDM-UHFFFAOYSA-N

Pictograms

Exclamation mark
GHS07

Signal Word

Warning

Hazard Classifications

Acute Tox. 4 Oral - Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
0000396476
0000363963
0000363963
0000353223
0000342803

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A simple and rapid method for the quantitation of proteins separated either by sodium dodecyl sulfate-electrophoresis or by isoelectric focusing in slab gels is presented. The method is based on the solubility of polyacrylamide gels crosslinked with N, N'-1, 2-dihydroxyethylenebisacrylamide
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Questions

1–2 of 2 Questions  

  1. How can I make 2% dheba for my expansion microscopy protocol? It seems that  it doesn’t dissolve easily in water. 

    1 answer

    1. This product is not tested for solubility.

      A literature search yielded the following: "Cross-linking agent for polyacrylamide gel electrophoresis that produces a gel which is soluble in dilute periodic acid or dilute aqueous solutions of bases". This information is from 'Polyacrylamide gels with modified cross-linkages' (P B O'Connell and C J Brady, Analytical biochemistry, 76(l), undefined (1976-11-1))

      There is also a recommendation to incubate at 50 °C for 16 hours until solubilized (in Periodic acid).

      Helpful?

  2. Where do you store the powder form of DHEBA? Does it need to be desiccated after opening?

    1 answer

    1. Storage conditions are at ambient temperatures in accordance with section 7 of the Safety Data Sheet.
      https://www.sigmaaldrich.com/sds/aldrich/294381?userType=new_user
      This product is not indicated as hygroscopic or moisture sensitive. Desiccator storage is not a requirement, but the reagent should be stored tightly closed under dry conditions.

      Helpful?

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