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A0521

Sigma-Aldrich

α-Amylase from human saliva

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Type IX-A, lyophilized powder, 1,000-3,000 units/mg protein

Synonym(s):

1,4-α-D-Glucan-glucanohydrolase

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About This Item

CAS Number:
9000-90-2
Enzyme Commission number:
3.2.1.1 (BRENDA, IUBMB)
EC Number:
232-565-6
MDL number:
MFCD00081319
UNSPSC Code:
12352204
eCl@ss:
32160410
NACRES:
NA.54

type

Type IX-A

Quality Level

200

form

lyophilized powder

specific activity

1,000-3,000 units/mg protein

composition

Protein, 5.0-15.0% biuret

greener alternative product characteristics

Waste Prevention
Design for Energy Efficiency
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sustainability

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UniProt accession no.

P0DUB6

greener alternative category

Enabling,

storage temp.

−20°C

Gene Information

human ... AMY1A(276)

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General description

α-amylase is the main secretory protein constituent of saliva. It contains 496 amino acids and has two isoforms, glycosylated and non-glycosylated. α-amylase gene is located on human chromosome 1p21.1. α-amylase is mainly produced in pancreas and saliva.
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Application

α-amylase from human saliva has been used to test α-amylase inhibitors using HPLC (high performance liquid chromatography). It also has been used to study the digestibility of human milk oligosaccharides (HMO).

Biochem/physiol Actions

α-amylase catalyses the hydrolysis of α-1,4 glycosidic linkage in oligosaccharides. It plays a crucial role in the initial digestion of starch, glycogen and polysaccharides. Elevated levels of amylase in serum is linked to acute pancreatitis.

Unit Definition

One unit will liberate 1.0 mg of maltose from starch in 3 min at pH 6.9 at 20 °C.

Physical form

Lyophilized powder containing (NH4)2SO4 and sodium citrate

Preparation Note

Chromatographically purified

Other Notes

View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer

substrate

Product No.
Description
Pricing

Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Statements

H334

Hazard Classifications

Resp. Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Structure of human salivary alpha-amylase crystallized in a C-centered monoclinic space group
Fisher SZ, et al.
Acta Crystallographica Section F, Structural Biology and Crystallization Communications, 62(2), 88-93 (2006)
Copy number polymorphism of the salivary amylase gene: implications in human nutrition research
Santos JL, et al.
Lifestyle Genomics, 5(3), 117-131 (2012)
M J Gnoth et al.
The Journal of nutrition, 130(12), 3014-3020 (2000-12-09)
In examining the functional aspects of human milk oligosaccharides (HMO), it is not known whether they are digested during the passage through the infant's gastrointestinal tract. HMO were prepared from individual milk samples (n = 6) and separated into neutral
M Alberta Araújo et al.
Biomaterials, 25(13), 2687-2693 (2004-01-31)
Apart from favourable physico-chemical and mechanical properties, the most important requirement for a biodegradable polymer to be used in medical applications is its biocompatibility and the non-cytotoxicity of its degradation products. Their combined effect should assure the safe material degradation
Katrin Stein et al.
The British journal of nutrition, 104(8), 1101-1111 (2010-06-29)
Dietary fibre is fermented by the human gut flora resulting mainly in the formation of SCFA, for example, acetate, propionate and butyrate. SCFA, in particular butyrate, may be important for secondary cancer prevention by inducing apoptosis and inhibiting cell growth
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Protocols

Enzymatic Assay of α-Amylase (EC 3.2.1.1)

Follow our procedure for the determination of alpha-Amylase activity. This enzymatic assay of a-Amylase guides you through the entire process and necessary calculations.

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