Mycoplasma PCR ELISA Protocol

Product No. 11663925910

Protocol Notes

Determination of lysed Mycoplasma

Dead and lysed Mycoplasma will have degraded DNA, therefore, it is likely that only live Mycoplasma with intact DNA will be detected.

Interupting the Mycoplasma PCR ELISA

The Mycoplasma PCR ELISA can be interrupted at the following steps:

After the PCR reaction, the reaction tube containing the PCR product can be stored overnight at 4 °C or for several weeks at -20 °C.

After hybridization of the PCR product to the capture oligo, the reaction tube can be stored at 4 °C.

Reading range for controls in the Mycoplasma PCR ELISA

At 405 nm, the following reading range is recommended:

Positive controls: 1.2 OD or higher
If the value for the positive control is less than 1.2 OD, the experiment should be repeated.  

Negative controls: 0-0.25 OD
If the value for the negative control is more than 0.25 OD, the experiment should be repeated.

Mycoplasma: species differentiation

Due to the generic PCR primers used in the Mycoplasma PCR ELISA Kit, it is not possible to differentiate Mycoplasma species. To differentiate the four most common Mycoplasma species, use Roche's Mycoplasma Detection Kit (11296744001).