UHPLC Analysis of Methapyrilene and Tripelennamine on Titan C18

column

Titan C18, 10 cm x 2. 1 mm I.D., 1.9 um particles (577124-U)

mobile phase

[A] 10 mM ammonium acetate, pH 7.0; [B] acetonitrile (70:30, A;B)

flow rate

0.3 mL/min

column temp.

35 °C

detector

MS, XIC m/z 262.14 and 256.18

injection

2 μL

sample

300 ng/mL each in acetonitrile:water (3:1)