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HPLC Analysis of Statins in Rat Plasma on Ascentis® Express C18 after SPE using Supel-Select HLB

HPLC Analysis of Statins in Rat Plasma on Ascentis® Express C18 after SPE using Supel™-Select HLB application for SPE, application for LC-MS

Materials

analytical column

Product No.
Description
Pricing

Ascentis® Express C18, 2.7 μm HPLC Column

2.7 μm particle size, L × I.D. 5 cm × 2.1 mm

SPE tube or plate

Product No.
Description
Pricing

Supel-Select HLB SPE Tube

bed wt. 30 mg, volume 1 mL, pk of 100

CONDITIONS

sample preparation

SPE (Solid Phase Extraction)

sample/matrix

rat plasma spiked with provastin and atorvastin at 5 and 100 ng/mL

SPE tube/cartridge

Supel-Select HLB SPE, 30 mg/1 mL (54181-U)

condition

0.5 mL methanol:acetonitrile (1:1, v/v); 0.5 mL DI water

sample addition

0.5 mL

washing

0.5 mL 5% methanol

elution

0.5 mL methanol:acetonitrile (1:1, v/v)

column

Ascentis Express C18, 5 cm x 2.1 mm, 2.7 μm particles (53822-U)

eluate post-treatment

evaporate to dryness with nitrogen at 37 °C, reconstitute in 0.5 mL DI water

mobile phase

0.1% acetic acid diluted in 60% methanol

flow rate

0.2 mL/min

column temp.

35 °C

detector

ABI 3200 QT; ESI(+), MRM (423.3/321.3, 423.3/101.0, 557.3/397.2 and 557.3/453.4)

injection

5 μL

Description

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany
Supel is a trademark of Sigma-Aldrich Co. LLC