HPLC Analysis of Metabolites of 7,12-Dimethylbenz[a]anthracene on SUPELCOSIL™ LC-18
Materials
Analytical column
Product No.
Description
Pricing
CONDITIONS
column
SUPELCOSIL LC-18, 25 cm × 4.6 mm I.D., 5 μm particles (58298)
mobile phase
[A] methanol:[B] water, (50:50, A:B), 10 min to 100:0 at 2.5% /min
flow rate
0.8 mL/min
detector
fluorescence
sample
rat liver, 9000 × g supernatant fraction from Aroclor-treated animals
Description
Legal Information
SUPELCOSIL is a trademark of Sigma-Aldrich Co. LLC