HPLC Analysis of Yeast Lysate on Proteomix^® SAX-NP3 Demonstrating High Separation Efficency

column temp.

25 °C

column

Proteomix SAX-NP3, 5 cm x 4.6 mm I.D., 3 μm particles (Z777219)

detector

UV, 280 nm

flow rate

0.5 mL/min

gradient

0 - 100% B in 30 min

injection

40 μL

mobile phase

(A) 20 mM Tris, pH 9.0; (B) A + 0.5 M NaCl

sample

2.5 mg/mL