HPLC Analysis of Milk Thistle Standards on Ascentis® Express C18
Materials
Analytical column
Product No.
Description
Pricing
CONDITIONS
column
Ascentis Express C18, 10 cm x 3 mm I.D., 2.7 μm particles (53814-U)
mobile phase
[A] water with 0.1% formic acid; [B] methanol
gradient
held at 35% B for 3 min; 35 to 45% B in 10 min; held at 45% B for 2 min; 45 to 100% B in 5 min
flow rate
0.6 mL/min
pressure
4105 psi (283 bar)
column temp.
35 °C
detector
UV, 254 nm
injection
20 μL
sample
20 μg/mL in 80:20, water:methanol
Description
Analysis Note
This application demonstrates the suitability of Ascentis Express C18 for the efficient separation of milk thistle related compounds.
Legal Information
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany