HPLC Analysis of Methionine Enantiomers on Astec® CHIROBIOTIC® T
Materials
Analytical column
Product No.
Description
Pricing
Guard cartridge
Product No.
Description
Pricing
Astec® CHIROBIOTIC® T Chiral HPLC Guard Column
Cartridge, 5 μm particle size, L × I.D. 2 cm × 4 mmstandard
Product No.
Description
Pricing
L-Methionine
from non-animal source, meets EP, JP, USP testing specifications, suitable for cell culture, 99.0-101.0%L-Methionine
certified reference material, TraceCERT®, Manufactured by: Sigma-Aldrich Production GmbH, SwitzerlandCONDITIONS
column
CHIROBIOTIC T, 25 cm x 4.6 mm I.D., 5 μm particles (12024AST)
mobile phase
(A) water; (B) methanol; (C) formic acid; (30:70:0.02, A:B:C)
flow rate
1 mL/min
column temp.
25 °C
detector
UV, 205 nm
injection
10 μL
sample
300 μg /mL in 30:70 water:methanol
Description
Legal Information
Astec is a registered trademark of Merck KGaA, Darmstadt, Germany
CHIROBIOTIC is a registered trademark of Sigma-Aldrich Co. LLC