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HPLC Analysis of Fat Soluble Vitamins by Normal Phase Chromatography on Ascentis® Si, 15 cm Column

HPLC Analysis of Fat Soluble Vitamins by Normal Phase Chromatography on Ascentis® Si, 15 cm Column application for HPLC

Materials

Analytical column

Product No.
Description
Pricing

581512-U

Ascentis® Si HPLC Column

5 μm particle size, L × I.D. 15 cm × 4.6 mm

standard

Product No.
Description
Pricing

47763

Cholecalciferol (D3)

analytical standard

47775

Menadione (K3)

analytical standard

47783

(±)-α-Tocopherol

analytical standard

47785

(+)-γ-Tocopherol

analytical standard

CONDITIONS

column

Ascentis Si, 15 cm × 4.6 mm I.D., 5 μm particles (581512-U)

mobile phase

[A] hexane, [B] ethyl acetate

gradient

10 to 30% B in 10 min; held for 2 min; to 10% B in 1 min

flow rate

1.0 mL/min

column temp.

30 °C

detector

UV, 290 nm

injection

10 μL

sample

as indicated in 96:4 (hexane:isopropanol)

Description

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

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