HPLC Analysis of Coumachlor Enantiomers on Astec^® CHIROBIOTIC^® V

column

CHIROBIOTIC V, 25 cm x 4.6 mm I.D., 5 μm particles (11024AST)

mobile phase

[A] 20 mM ammonium nitrate, pH 5.5; [B] THF; (75:25, A:B)

flow rate

0.75 mL/min

column temp.

25 °C

detector

UV, 254 nm

injection

2 μL

sample

2.5 mg/mL in methanol