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HPLC Analysis of Cetirizine and Hydroxyzine on Ascentis® Express F5

HPLC Analysis of Cetirizine and Hydroxyzine on Ascentis® Express F5 application for HPLC

Materials

Analytical column

Product No.
Description
Pricing

53578-U

Ascentis® Express F5, 2.7 μm HPLC Column

2.7 μm particle size, L × I.D. 10 cm × 3 mm

Standard

Product No.
Description
Pricing

C-158

Cetirizine dihydrochloride solution

1.0 mg/mL in acetonitrile: water (9:1) (as free base), ampule of 1 mL, certified reference material, Cerilliant®

H-124

Hydroxyzine dihydrochloride solution

1.0 mg/mL in methanol (as free base), ampule of 1 mL, certified reference material, Cerilliant®

CONDITIONS

column

Ascentis Express F5, 10 cm x 3.0 mm I.D., 2.7 μm particles (53578-U)

mobile phase

5 mM ammonium formate pH 3.0 with formic acid in 90:10, acetonitrile:water

flow rate

0.6 mL/min

pressure

1300 psi (90 bar)

column temp.

25 °C

detector

UV, 254 nm

injection

2 μL

sample

50 μg/mL in 90:10, acetonitrile:water

Description

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

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