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HPLC Analysis of Barbiturates on Discovery® C18

HPLC Analysis of Barbiturates on Discovery® C18 application for HPLC

Materials

Analytical column

Product No.
Description
Pricing

SUPELCOSIL LC-ABZ HPLC Column

5 μm particle size, L × I.D. 15 cm × 4.6 mm

CONDITIONS

column

Discovery C18, 15 cm × 4.6 mm I.D., 5 μm particles (504955)

mobile phase

[A] acetonitrile; [B] 25 mM potassium phosphate buffer, pH 6.9 (25:75, A:B)

flow rate

1.5 mL/min

column temp.

ambient

detector

UV, 254 nm

injection

25 μL

Description

Legal Information

Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany