HPLC Analysis of Alanine Enantiomers on Astec^® CHIROBIOTIC^® TAG

column

CHIROBIOTIC TAG, 25 cm x 4.6 mm I.D., 5 μm particles (14024AST)

mobile phase

[A] water; [B] acetonitrile; (50:50, A:B)

flow rate

0.8 mL/min

column temp.

25 °C

detector

UV, 210 nm

injection

2 μL

sample

5 mg/mL in methanol