GC Analysis of Volatiles in White Wine on VOCOL® after SPME using a 75 μm Carboxen/PDMS Fiber
Materials
Analytical column
SPME fiber
SPME fiber assembly Carboxen/Polydimethylsiloxane (CAR/PDMS)
df 75 μm(CAR/PDMS, for use with manual holder, needle size 24 gaStandard
mobile phase component
CONDITIONS
sample/matrix
white wine + 25% NaCl
SPME fiber
Carboxen/PDMS, 75 μm (57318)
extraction
10 min, 40 °C (headspace)
desorption process
3 min at 290 °C
column
VOCOL, 30 m × 0.25 mm I.D., 1.5 μm (24205-U)
oven
40 °C (2 min) to 220 °C at 10 °C/min
inj. temp.
290 °C
detector
GC-MS, quadrupole, m/z = 31-240
carrier gas
helium, 35 cm/sec
injection
splitless/split, closed 1 min
liner
0.75 mm liner
sample
white wine
Description
Analysis Note
In this application, SPME was used for the analysis of volatiles in white wine using a headspace method. The presence of aroma compounds in white wine is one of the most important attributes of its final quality. A Carboxen/PDMS fiber was inserted into the headspace of the vial for 10 minutes at 40 °C and analyzed by GC/MS. The thick film of the VOCOL GC column helps focus the volatile analytes.
Legal Information
VOCOL is a registered trademark of Merck KGaA, Darmstadt, Germany