- Non-radioactive LATS in vitro Kinase Assay.
Non-radioactive LATS in vitro Kinase Assay.
Bio-protocol (2017-11-07)
Audrey W Hong, Kun-Liang Guan
PMID29104896
ABSTRACT
This protocol describes a method to directly measure LATS activity by an in vitro kinase assay using YAP as a substrate.
MATERIALS
Product Number
Brand
Product Description
Sigma-Aldrich
Anti-Glutathione-S-transferase (GST) antibody, Mouse monoclonal, clone 2H3-D10, purified from hybridoma cell culture