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Cy3B: improving the performance of cyanine dyes.

Journal of fluorescence (2004-12-24)
Michael Cooper, Andreas Ebner, Mark Briggs, Miles Burrows, Nicholas Gardner, Robert Richardson, Richard West
ABSTRACT

The spectral properties of a rigidified trimethine cyanine dye, Cy3B have been characterised. This probe has excellent fluorescent properties, good water solubility and can be bioconjugated. The emission properties of this fluorophore have also been investigated upon conjugation to an antibody. This study compared the conjugated emission properties of Cy3B with other commercially available fluorophores emitting at similar wavelengths.

MATERIALS
Product Number
Brand
Product Description

Supelco
Atto 565 NHS ester, BioReagent, suitable for fluorescence, ≥90% (HPLC)