Skip to Content
MilliporeSigma

Application of the Fenton process in the elimination of helminth eggs.

Journal of water and health (2014-12-05)
Sandra Isabel Escobar-Megchún, Hugo Alejandro Nájera-Aguilar, Magaly González-Hilerio, Javier Gutiérrez-Jiménez, Rubén Fernando Gutiérrez-Hernández, María Neftalí Rojas-Valencia
PMID25473981
ABSTRACT

This study relates to a method for evaluating the degradation efficiency of Ascaris lumbricoides eggs through a Fenton reaction, using hydrogen peroxide (H2O2) at 100, 250, and 500 mg/L, ferrous sulfate (FeSO4) at 458 mg/L Fe(2+) and pH values of 3, 4.5, and 6. The experiments were conducted according to a 3(2) experimental design, with 1:1, 3:1, and 5:1 (H2O2/Fe(2+)) molar ratios. The oxidation and flocculation stages were performed at 130 rpm during 2 hours and at 25-30 rpm during 20 min, respectively. As a result of the Fenton reaction, an average of 91.2% Ascaris lumbricoides egg degradation was achieved at pH 6 using a 500 mg/L dose of H2O2 and a 3:1 (H2O2/Fe(2+)) molar ratio. Thus, this process is an alternative for eliminating parasites that are resistant to conventional disinfection processes and significantly reduces the human health hazard they represent.

MATERIALS
Product Number
Brand
Product Description
316989
Sigma-Aldrich
Hydrogen peroxide solution, contains potassium stannate as inhibitor, 30-32 wt. % in water, semiconductor grade, 99.999% trace metals basis
209309
Sigma-Aldrich
Iron, powder, −325 mesh, 97%
IRMM524B
Iron, IRMM®, certified reference material, 0.5 mm wire
18304
Sigma-Aldrich
Hydrogen peroxide solution, 34.5-36.5%
95321
Supelco
Hydrogen peroxide solution, ≥30%, for trace analysis
95294
Sigma-Aldrich
Hydrogen peroxide solution, tested according to Ph. Eur.
88597
Millipore
Hydrogen peroxide solution, 3%, suitable for microbiology
267945
Sigma-Aldrich
Iron, chips, 99.98% trace metals basis
356808
Sigma-Aldrich
Iron, foil, thickness 0.1 mm, ≥99.9% trace metals basis
F8263
Sigma-Aldrich
Iron(II) sulfate heptahydrate, suitable for plant cell culture, ≥99%
H1009
Sigma-Aldrich
Hydrogen peroxide solution, 30 % (w/w) in H2O, contains stabilizer
F8633
Sigma-Aldrich
Iron(II) sulfate heptahydrate, BioReagent, suitable for cell culture, suitable for insect cell culture, ≥99%
44890
Sigma-Aldrich
Iron, puriss. p.a., carbonyl-Iron powder, low in magnesium and manganese compounds, ≥99.5% (RT)
15-1490
Sigma-Aldrich
Iron(II) sulfate heptahydrate, JIS special grade, 99.0-102.0%
13-1920
Sigma-Aldrich
Hydrogen peroxide solution, SAJ first grade, ≥30.0%
16911
Supelco
Hydrogen peroxide solution, 30 % (w/w), for ultratrace analysis
GF55461539
Iron, rod, 1000mm, diameter 30mm, as drawn, 98+%
GF27009514
Iron, rod, 150mm, diameter 5.0mm, as drawn, 99.99+%
GF56648313
Iron, foil, 100x100mm, thickness 0.15mm, hard, 99.5%
GF41714906
Iron, rod, 200mm, diameter 8.0mm, as drawn, 99.99+%
GF52769807
Iron, foil, light tested, 50x50mm, thickness 0.025mm, hard, 99.5%
GF33971283
Iron, foil, light tested, 25x25mm, thickness 0.0125mm, 99.99+%
GF88665452
Iron, rod, 100mm, diameter 4.8mm, as drawn, 98+%
GF15623917
Iron, foil, not light tested, 50x50mm, thickness 0.002mm, 99.85%
GF52254233
Iron, rod, 100mm, diameter 100mm, as drawn, armcO« soft ingot 99.8%
GF12054024
Iron, rod, 500mm, diameter 19mm, as drawn, soft ingot 99.8%
GF93388999
Iron, foil, light tested, 25x25mm, thickness 0.075mm, hard, 99.5%
GF48449391
Iron, rod, 1000mm, diameter 12.7mm, as drawn, 98+%
GF73036795
Iron, foil, 50mm disks, thickness 0.38mm, hard, 99.5%
GF52296419
Iron, rod, 500mm, diameter 40mm, as drawn, armcO« soft ingot 99.8%