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The histidine kinase CusS senses silver ions through direct binding by its sensor domain.

The histidine kinase CusS senses silver ions through direct binding by its sensor domain.

Biochimica et biophysica acta (2014-06-21)

Swapna A Gudipaty, Megan M McEvoy

ABSTRACT

The Cus system of Escherichia coli aids in protection of cells from high concentrations of Ag(I) and Cu(I). The histidine kinase CusS of the CusRS two-component system functions as a Ag(I)/Cu(I)-responsive sensor kinase and is essential for induction of the genes encoding the CusCFBA efflux pump. In this study, we have examined the molecular features of the sensor domain of CusS in order to understand how a metal-responsive histidine kinase senses specific metal ions. We find that the predicted periplasmic sensor domain of CusS directly interacts with Ag(I) ions and undergoes a conformational change upon metal binding. Metal binding also enhances the tendency of the domain to dimerize. These findings suggest a model for activation of the histidine kinase through metal binding events in the periplasmic sensor domain.

MATERIALS

Product Number

Brand

Product Description

Silver, foil, 15mm disks, thickness 1.0mm, as rolled, 99.95+%

Copper, tube, 500mm, outside diameter 2.5mm, inside diameter 1.9mm, wall thickness 0.3mm, as drawn, 99.9%

Copper - O.F.H.C., foil, 300x300mm, thickness, 0.5mm, half hard, flattened, 99.95+%

Copper - O.F.H.C., foil, 4mm disks, thickness 0.010mm, hard, 99.95+%

Copper - O.F.H.C., foil, 4mm disks, thickness 0.025mm, hard, 99.95+%

Copper - O.F.H.C., foil, 6mm disks, thickness 0.075mm, half hard, 99.95+%

Silver, foil, 6mm disks, thickness 0.125mm, annealed, 99.95+%

Copper - O.F.H.C., foil, 2m coil, thickness 0.20mm, hard, 99.95+%

Copper - O.F.H.C., foil, 5m coil, thickness 0.075mm, half hard, 99.95+%

Copper - O.F.H.C., foil, 50mm disks, thickness 0.05mm, hard, 99.95+%

Copper - O.F.H.C., foil, 25mm disks, thickness 1.0mm, half hard, 99.95+%

Silver, foil, 25mm disks, thickness 1.0mm, as rolled, 99.95+%

Copper, wire reel, 100m, diameter 0.5mm, as drawn, 99.98+%

Copper - O.F.H.C., foil, 6mm disks, thickness 0.006mm, hard, 99.95+%

Copper, wire reel, 1m, diameter 0.75mm, as drawn, 99.98+%

Silver, foil, 6mm disks, thickness 0.125mm, as rolled, 99.95+%

Silver, foil, 6mm disks, thickness 0.0025mm, 99.97%

Copper - O.F.H.C., foil, 5m coil, thickness 0.125mm, hard, 99.95+%

Copper - O.F.H.C., foil, 50mm disks, thickness 0.075mm, half hard, 99.95+%

Silver, foil, light tested, 100x100mm, thickness 0.02mm, 99.97%

Copper - O.F.H.C., foil, 50mm disks, thickness 0.25mm, half hard, 99.95+%

Copper, wire reel, 100m, diameter 1.0mm, as drawn, 99.98+%

Copper, wire reel, 100m, diameter 0.025mm, as drawn, 99.99+%

Silver, foil, 6mm disks, thickness 0.015mm, 99.97%

Silver, foil, 5m coil, thickness 0.0125mm, coil width 9mm, 99.95+%

Copper, wire reel, 0.5m, diameter 0.5mm, as drawn, 99.999%

Copper, wire reel, 100m, diameter 0.02mm, hard, 99.99+%

Copper, wire reel, 500m, diameter 0.05mm, annealed, 99.9%

Copper, wire reel, 1m, diameter 0.01mm, hard, 99.99%

Copper, wire reel, 2000m, diameter 0.05mm, annealed, 99.9%