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  • [The influence of carrageenan, colchicine, 2,4-dinitrophenol, ethanol, galactose, indomethacin and liquoid on hemolytic complement activity (CH50E)].

[The influence of carrageenan, colchicine, 2,4-dinitrophenol, ethanol, galactose, indomethacin and liquoid on hemolytic complement activity (CH50E)].

Allergie und Immunologie (1986-01-01)
A Stelzner, H Schleef
ABSTRACT

The influence of Liquoid (0.00005, 0.00001%), Carrageenan (0.001% up to 0.000001%), Colchicine (0.01% up to 0.0025%), Indomethacin (1 X 10(-4) up to 2.5 X 10(-5) mol/1), Ethanol (10% up to 1.25%), Galactose (1 X 10(-1) up to 1 X 10(-2) mol/l) and 2.4-Dinitrophenol (5 X 10(-4) up to 1 X 10(-5) mol/l) on the total hemolytic activity of complement was tested. By Liquoid, Carrageenan, Colchicine and Ethanol in all studied concentrations an remarkable inhibition of the CH50E could be estimated. Indomethacin up to an concentration of 3.75 X 10(-5) mol/l reduced the activity partially. In contrast, Galactose and 2.4-Dinitrophenol have not influenced the complement titers.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Polyanetholesulfonic acid sodium salt
Sigma-Aldrich
Polyanetholesulfonic acid sodium salt, BioUltra
Sigma-Aldrich
Polyanetholesulfonic acid sodium salt, powder