[Determination of orientin and vitexin in Trollius chinesis preparation by HPLC].

To establish a HPLC method for determining orientin and vitexin in Trollius chinesis preparation. The separation was carried out on Hypersil ODS column with a mixture of acetonitrile-1% acetic acid solution (15:85) as the mobile phase, at a flow rate of 1.0 mL x min(-1). The detection wavelength was set 340 nm. Orientin showed a good linear relationship in the range of 8.42-84.2 microg x mL(-1) (r = 0.9999) and the average recovery is 98.3%, RSD is 0.8%. Vitexin showed a good linear relationship in the range of 8.07-80.7 microg x mL(-1) (r = 0.9999) and the average recovery is 102.4%. RSD is 1.7%. The method is sensitive, specific, and accurate with a good repeatability and can be used for the quality control of Trollius chinesis preparation.