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  • BRCA1 affects lipid synthesis through its interaction with acetyl-CoA carboxylase.

BRCA1 affects lipid synthesis through its interaction with acetyl-CoA carboxylase.

The Journal of biological chemistry (2005-12-06)
Karen Moreau, Eva Dizin, Hind Ray, Céline Luquain, Etienne Lefai, Fabienne Foufelle, Marc Billaud, Gilbert M Lenoir, Nicole Dalla Venezia
ABSTRACT

Germ line alterations in BRCA1 (breast cancer susceptibility gene 1) are associated with an increased susceptibility to breast and ovarian cancer. BRCA1 acts as a scaffold protein implicated in multiple cellular functions, such as transcription, DNA repair, and ubiquitination. However, the molecular mechanisms responsible for tumorigenesis are not yet fully understood. We have recently demonstrated that BRCA1 interacts in vivo with acetyl coenzyme A carboxylase alpha (ACCA) through its tandem of BRCA1 C terminus (BRCT) domains. To understand the biological function of the BRCA1.ACCA complex, we sought to determine whether BRCA1 is a regulator of lipogenesis through its interaction with ACCA. We showed here that RNA inhibition-mediated down-regulation of BRCA1 expression induced a marked increase in the fatty acid synthesis. We then delineated the biochemical characteristics of the complex and found that BRCA1 interacts solely with the phosphorylated and inactive form of ACCA (P-ACCA). Finally, we demonstrated that BRCA1 affects lipid synthesis by preventing P-ACCA dephosphorylation. These results suggest that BRCA1 affects lipogenesis through binding to P-ACCA, providing a new mechanism by which BRCA1 may exert a tumor suppressor function.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Acetyl-CoA carboxylase 2 human, recombinant, expressed in Sf9 cells
Sigma-Aldrich
Acetyl-CoA Carboxylase 1 human, recombinant, expressed in Sf9 cells
Sigma-Aldrich
Anti-BRCA1 (Ab-1) Mouse mAb (MS110), liquid, clone MS110, Calbiochem®