- Osteogenic Differentiation from Mouse Embryonic Stem Cells.
Osteogenic Differentiation from Mouse Embryonic Stem Cells.
Methods in molecular biology (Clifton, N.J.) (2021-10-07)
Zahra Alvandi, Michal Opas
PMID34611818
ABSTRACT
Embryonic stem cells (ESCs) are a unique model that allows the study of molecular pathways underlying commitment and differentiation. We have studied signaling pathways and their contributions to osteogenic differentiation. In addition to our previously published protocol where we recommended the addition of retinoic acid with later addition of dexamethasone to boost osteogenic lineage cells, here we describe an optimized protocol for osteogenic differentiation from R1 ESCs with suggestions for inhibition of Src activity.
MATERIALS
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Sigma-Aldrich
PP2, PP2, CAS 172889-27-9, is a potent, reversible, ATP-competitive, inhibitor of the Src family of protein tyrosine kinases (IC₅₀ = 4, 5, 5, &100 nM for p56lck, p59fynT, Hck, & Src, respectively).