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  • Recombinant human basic fibroblast growth factor (rhbFGF) induces secretion of nerve growth factor (NGF) in cultured rat astroglial cells.

Recombinant human basic fibroblast growth factor (rhbFGF) induces secretion of nerve growth factor (NGF) in cultured rat astroglial cells.

Neuroscience letters (1991-01-28)
H Fukumoto, M Kakihana, M Suno
ABSTRACT

Recombinant human basic fibroblast growth factor (rhbFGF) was found to induce secretion of nerve growth factor (NGF) in cultured astrocytes from rat brain. The induction was concentration dependent; a maximum increase of 2-fold was observed at concentrations of 3-10 ng/ml or rhbFGF. When rhbFGF was added to the astrocytes, the induction occurred within 8 h and leveled off after 12 h. Antiserum against rhbFGF completely blocked the induction. Maximum induction occurred under serum-free culture conditions and in cultures with a high cell density. These results suggest that rhbFGF may exert a neurotrophic effect on the central nervous system through the induction of NGF secretion by astrocytes.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
NGF-β human, from human, recombinant, expressed in NSO cells, lyophilized powder, suitable for cell culture
Sigma-Aldrich
Fibroblast Growth Factor-Basic Heparin Stabilized human, FGF-Basic, recombinant, expressed in E. coli, sterile-filtered, aqueous solution, suitable for cell culture