- Cleavage of L-leucine-containing dipeptides by Clostridium butyricum.
Cleavage of L-leucine-containing dipeptides by Clostridium butyricum.
The ability of Clostridium butyricum cultures to hydrolyze three L-leucine-containing dipeptides (Leu-Leu, Leu-Gly and Gly-Leu) in a synthetic minimal medium is demonstrated by using gas chromatography coupled with mass spectrometry. The 13C nuclear magnetic resonance and a labeled dipeptide L-[1-13C]Leu-Gly were used to confirm this activity.