G1007512
UHPLC/MS Analysis of Insulin Analogs on BIOshell A160 Peptide C18
application for UHPLC-MS
About This Item
technique(s)
UHPLC-MS: suitable
test parameters
column: BIOshell A160 Peptide C18, 15 cm x 2.1 mm I.D., 2.0 μm particles (67243-U)
mobile phase: [A] 75:25 (0.1% DFA in water: 0.1% DFA in acetonitrile); [B] 50:50 (0.1% DFA in water: 0.1% DFA in acetonitrile)
gradient: 23% B for 7 min; 23% B to 100% B in 1 min; hold at 100% B for 7 min
flow rate: 0.2 mL/min
pressure: 2500 psi (172 bar)
column temp.: 75 °C
detector: ESI(+), TIC 100-3000 m/z
injection: 0.5 μL
sample: Analog Mixture, 20 μg/mL, 6:3:1 methanol:water:acetic acid containing 0.1% rat plasma
suitability
application for UHPLC-MS
application(s)
life science and biopharma
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