G005911

LC/MS Analysis of Illicit Bath Salts on Ascentis^® Express HILIC, Set 2

application for HPLC

Properties

• technique(s): HPLC: suitable
• test parameters: column: Ascentis Express HILIC, 10 cm x 2.1 mm I.D., 2.7 μm particles (53939-U); mobile phase: [A] 5 mM ammonium formate in acetonitrile: [B] water; (98:2, A:B); flow rate: 0.6 mL/min; pressure: 267 psi (18.4 bar); column temp.: 35 °C; detector: MS, ESI(+) TOF, Summed Ion Chromatogram; injection: 2 μL; sample: 500 ng/mL in acetonitrile
• suitability: application for HPLC
• application(s): forensics and toxicology

Description

Analysis Note

HILIC mode operation on an Ascentis Express column provided baseline resolution of these bath salts, including isobaric compounds. The highest quality LC-MS solvents were used to supply low background interference and low particulate contaminants for robust; trouble-free operation. Cerilliant CRMs provided reliable identification and quantification.

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

Analyte

α-Pyrrolidinovalerophenone hydrochloride solution, 1.0 mg/mL in methanol (as free base), ampule of 1 mL, certified reference material, Cerilliant^®
3,4-Methylenedioxypyrovalerone HCl (MDPV) solution, 1.0 mg/mL in methanol (as free base), ampule of 1 mL, certified reference material, Cerilliant^®
Butylone hydrochloride solution, 1.0 mg/mL in methanol (as free base), ampule of 1 mL, certified reference material, Cerilliant^®
Ethylone hydrochloride, 1.0 mg/mL in methanol (as free base), ampule of 1 mL, certified reference material, Cerilliant^®
Buphedrone ephedrine metabolite hydrochloride solution, 1 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant^®
(±)-4-Methylephedrine hydrochloride solution, 1.0 mg/mL in methanol (as free base), ampule of 1 mL, certified reference material, Cerilliant^®