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G005861

HPLC Analysis of Fat-Soluble Vitamins on Ascentis® Express C18, Methanol Mobile Phase

application for HPLC

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About This Item

technique(s)

HPLC: suitable

test parameters

column: Ascentis Express C18, 15 cm x 4.6 mm I.D., 2.7 μm particles (53829-U)
mobile phase: methanol
flow rate: 1.5 mL/min
pressure: 3249 psi (224 bar)
column temp.: 30 °C
detector: UV, 280 nm
injection: 5 μL
sample: 0.5 mg/mL tocopherol acetate, alpha-tocopherol, delta-tocopherol and K1, 0.05 mg/mL D2, D3 and retinol acetate, 1.5 mg/mL retinol palmitate, all in ethanol.

suitability

application for HPLC

application(s)

vitamins, nutraceuticals, and natural products

Analysis Note

Analysis was conducted using standard solution, detection via UV.

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

Analyte

Description
    Retinyl acetate, analytical standard
    δ-Tocopherol, analytical standard
    Ergocalciferol (D2), analytical standard
    Cholecalciferol (D3), analytical standard
    (±)-α-Tocopherol, analytical standard
    DL-alpha-Tocopherol acetate, analytical standard
    Phylloquinone (K1), analytical standard
    Retinol palmitate, analytical standard
    See All (8)

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