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G005720

HPLC Analysis of Phenylacetic Acid on Ascentis® C18

application for HPLC

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About This Item

technique(s)

HPLC: suitable

test parameters

column: Ascentis C18, 15 cm x 4.6 mm I.D., 5 μm particles (581324-U)
mobile phase: [A] 20 mM phosphoric acid; [B] acetonitrile; (75:25, A:B)
flow rate: 1.0 mL/min
column temp.: 35 °C
detector: UV, 215 nm
injection: 5 μL
sample: 50 μg/mL in water: acetonitrile: methanol (75: 20: 5)

suitability

application for HPLC

application(s)

chemicals and industrial polymers

Analysis Note

This application demonstrates the suitability of Ascentis C18 for the analysis of phenylacetic acid.

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

Analyte

Description
    Phenylacetic acid

analytical column

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Description
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standard

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