G005607
LC-MS/MS Analysis of Melamine Extracted from Milk on Ascentis® Express HILIC
application for HPLC
About This Item
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technique(s)
HPLC: suitable
test parameters
column: Ascentis Express HILIC, 10 cm x 2.1 mm I.D., 2.7 μm particles (53939-U)
mobile phase: [A] 10 mM ammonium formate in 10:90 (v/v) water:acetonitrile; [B] 10 mM ammonium formate in 30:70 (v/v) water:acetonitrile
gradient: held at 0% B for 5 min; 0 to 100% B in 0.1 min; held at 100% B for 4.9 min; 100 to 0% B in 0.1 min; held at 0% B for 4.9 min
flow rate: 0.2 mL/min
column temp.: 30 °C
detector: ABI 3200 Q-Trap
injection: 2 μL
sample: SPE extract from spiked milk
suitability
application for HPLC
application(s)
food and beverages
Legal Information
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Analytical column
Standard
mobile phase component
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