823483

TSKgel^® Protein A-5PW (20 µm) HPLC Columns

L x I.D. 3.5 cm × 4.6 mm, Protein A phase

• UNSPSC Code: 41115709

Properties

• Product Name: TSKgel^® Protein A-5PW HPLC Column, 20 μm particle size, L × I.D. 3.5 cm × 4.6 mm
• material: PEEK
• product line: TSKgel^®
• manufacturer/tradename: TSKgel^®
• parameter: 20 bar pressure (290 psi); 4-30 °C temperature
• technique(s): HPLC: suitable
• L × I.D.: 3.5 cm × 4.6 mm
• matrix: polymeric particle platform
• matrix active group: Protein A phase
• particle size: 20 μm
• separation technique: affinity
• storage temp.: 2-8°C

Description

General description

TSKgel Protein A-5PW is a 20 μm, 4.6 mm ID × 3.5 cm column for high performance affinity chromatography. Made of PEEK hardware, this column has been designed for the rapid separation and robust quantification of a
variety of antibodies. Monoclonal antibodies from harvested cell culture media can be captured and accurately quantitated in less than 2 minutes per injection.

The TSKgel Protein A-5PW column can be used for more than 2,000 injections without regeneration or cleaning. Packed with hydroxylated methacrylic polymer beads, this column is designed with a high degree
of crosslinking, which allows a high flow rate for chromatography while still maintaining chromatographic efficiency, peak width and resolution. The recombinant protein A ligand is a code-modified hexamer of the C domain.
An enhanced rProtein A ligand is bound to the TSKgel 5PW base bead via multipoint attachment resulting in excellent base stability in 0.1 mol/L NaOH.

Footnote

Highlights

• Wide dynamic range with high sample concentration load for mAb quantitation
• Fast analysis: 1-2 min/analysis
• High sensitivity for mAb determination
• Long lifetime: .2,000 injections per column with no sign of deterioration

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Legal Information

TSKgel is a registered trademark of Tosoh Corporation