66905-U
BIOshell A160 Peptide C18 (2.7 μm) HPLC Columns
L × I.D. 15 cm × 2.1 mm, HPLC Column
Synonym(s):
Superficially Porous Wide Pore C18 UHPLC Column
About This Item
Recommended Products
product name
BIOshell A160 Peptide C18, 2.7 μm HPLC Column, 2.7 μm particle size, L × I.D. 15 cm × 2.1 mm
material
stainless steel hardware
Quality Level
agency
suitable for USP L1
description
Shell thickness (0.5 μm)
Solid Core (1.7 μm)
product line
BIOshell
BIOshell
feature
endcapped: no
analyte chemical class(es)
peptides
peptides
manufacturer/tradename
BIOshell
BIOshell
packaging
1 ea of
extent of labeling
4.6% carbon loading
parameter
600 bar max. pressure
90 °C max. temp.
technique(s)
HPLC: suitable
HPLC: suitable
LC/MS: suitable
LC/MS: suitable
UHPLC-MS: suitable
UHPLC-MS: suitable
UHPLC: suitable
UHPLC: suitable
L × I.D.
15 cm × 2.1 mm
surface area
90 m2/g
surface coverage
2.2 μmol/m2
matrix
spherical silica particle platform
superficially porous particle
superficially porous particle
matrix active group
C18 (octadecyl) bonding phase, diisobutyloctadecyl
particle size
2.7 μm
2.7 μm
pore size
160 Å
operating pH range
1-8
separation technique
reversed phase
reversed phase
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General description
application
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This application note compares three different column chemistries from the BIOshell™ line of columns with superficially porous particles to evaluate their performance in peptide mapping.
LC-UV-MS workflow details teriparatide peptide mapping, including enzymatic digestion, separation conditions, and QTOF mass spectrometer identification.
Method development for protein fingerprinting of AAV serotype 5 using both intact mass analysis and peptide mapping, to determine critical quality attributes for gene therapy, utilizing three different columns.
Choose Ascentis® Express Peptide ES-C18 U/HPLC Columns based on Fused-Core® technology for fast and efficient separation of high-molecular weight compounds, such as peptides and small proteins. With a 2.7 µm particle size and rigorous testing, these columns offer reliable results and high efficiency.
Protocols
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