Methanolic HCl (hydrochloric acid in methanol) is particularly useful for preparing methyl esters of volatile (short chain) fatty acids. Fatty acids are esterified by heating them with an anhydrous alcohol (e.g., methanol) in the presence of an acidic catalyst (e.g., HCl) in a sealed vessel at a high temperature for a short time. In the reaction, a fatty acid molecule and an alcohol molecule are joined, with the release of a water molecule. The derivatives can be quickly and easily recovered, quantitatively, from the reaction medium.
Features and Benefits
Derivatization of fatty acids, particularly volatile (short chain) fatty acids.
Clean reaction (no side reaction) with volatile by-products.
The Journal of biological chemistry, 276(34), 31561-31566 (2001-06-09)
The existence of Delta 4 fatty acid desaturation in the biosynthesis of docosahexanoic acid (DHA) has been questioned over the years. In this report we describe the identification from Thraustochytrium sp. of two cDNAs, Fad4 and Fad5, coding for Delta
Mitochondrial Function, Part B: Mitochondrial Protein Kinases, Protein Phosphatases and Mitochondrial Diseases.
William SA and Murphy AN.
Science, 88-89 (2009)
Practical Gas Chromatography: A Comprehensive Reference.
Wilder KD and Engewald W.
Science, 613-614 (2014)
Enamel color modifications by controlled hydrochloric acid pumice abrasion: a review with case summaries.
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